The Round of 16 kicks off in Paris tomorrow at 17.00 UK time (16.00 GMT).
Alessandro Florenzi is stepping in to his role on the right, but there could be more surprises in store.
Spain (probable): De Gea; Juanfran, Pique, Ramo在线外围投注s, Alba; Fabregas, Busquets, Iniesta; David Silva, Morata, Nolito
This would be why Matteo Darmian and Mattia De Sciglio are the favourites to take the left wing-back role in a 3-5-2, as they can help out more in midfield than Stephan El Shaarawy if it moved to 3-4-3.
It'd also pave the way for El Shaarawy or Lorenzo Insigne to come off the bench and harass a tiring Spain in the second half.
Antonio 21点官网Candreva is out of action for this match and accordin澳门外围投注g to recent reports could fail to recover even if Italy go all the way at Euro 2016.
Italy could mutate their 3-5-2 system into 3-4-3 with Emanuele Giaccherini moving up against Spain.
There are rumours that Antonio Conte could field a mobile and mutating side with Giaccherini either in midfield or pushing up to the wide right of a trident attack.
Italy (probable): Buffon; Barzagli, Bonucci, Chiellini; Florenzi, Parolo, De Rossi, Darmian; Eder, Pellè, Giaccherini
Eder can go wide left, leaving Graziano Pellè as a traditional centre-forward.